Thursday, 11 June 2015
VCRILIBRARY,NAMAKKAL: OPEN ACCESS WILEY VETERINARY E jOURNALS
VCRILIBRARY,NAMAKKAL: OPEN ACCESS WILEY VETERINARY E jOURNALS: OPEN ACCESS WILEY E JOURNALS 24 X 7 X 365 FROM ANYWHERE, ANY TIME, ANY ONE CAN ACCESS Anatomia, ...
Veterinary Parasitology procedures
FECAL OCCULT BLOOD
Purpose
The Hemoccult® test is a rapid, convenient and
qualitative method for detecting fecal occult blood which may be indicative of
gastrointestinal disease. It is not a
test for colorectal cancer or any other specific diseases.
Principle
The Hemoccult® test is based on the oxidation of guaiac
by hydrogen peroxide to a blue-colored compound. The heme portion of hemoglobin, if present in
the fecal specimen, has peroxidase activity which catalyzes the oxidation of alpha
guaiaconic acid (active component of the guaiac paper) by hydrogen peroxide
(active component of the developer) to form a highly conjugated blue quinine
compound.
Reagents/Materials
Hemoccult® slide test cards
Hemoccult® developer
Procedure
1. Label the front of the slide with:
• date
• medical record number
• animal’s name
2. Preparing the test
• using applicator
provided, collect small fecal sample
• apply a thin smear
covering Box A
• reuse applicator to
obtain second sample from a different part of stool
• apply a thin smear
covering Box B
• dispose of applicator in
waste container
• close cover flap
• wait 3-5 minutes before developing
3. Developing the test
• open back of
slide
• apply two
drops of Hemoccult® Developer to guaiac paper directly over
each smear
• read results within 60 seconds
• results – any trace of
blue on or at the edge of the smear is positive for occult blood
4. Developing the Performance
Monitor® feature (quality control)
• the Performance Monitor®
areas must be developed on every slide
• apply one drop
of Hemoccult® Developer between the positive and negative
Performance Monitor® areas
• read results within 10 seconds
• if the
slide/developer are functional, a blue color will appear in the positive
Performance Monitor® area and no blue will appear in the negative
Performance Monitor® area
• neither the
intensity nor the shade of the blue from the positive Performance Monitor®
area should be used as a reference for the appearance of positive test results
• any blue
originating from the positive Performance Monitor® area should be
ignored when reading the sample test results
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